Antibody Customer Review for p53 Polyclonal Antibody (STJ94890)


Target information

p53 is an important protein in the development of many cancers, acting as a tumour suppressor protein in many tumour types, inducing growth arrest or apoptosis depending on the physiological circumstances and cell type.

p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

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1. HOW DID OUR ANTIBODY PERFORM? ANTIBODY CUSTOMER REVIEW: p53 Polyclonal Antibody (STJ94890)
2. What was used? Primary antibody: p53 Antibody (STJ94890) Provider: St John’s Laboratory Dilution ratio: 1:500 Application: Western Blot Materials for validation: HepG2, HeLa, C6 and NIH3T3 cell lines Click for product data sheet PDF
3. What was the protocol? Primary Antibody Application 1:500 primary antibody was incubated at 4?C for overnight. Secondary Antibody Application Goat secondary antibody at 1:5000 for 1 hour at room temperature. Visualisation ECL kit. Exposure time 1 min.
4. What were the results?
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