No products in the cart.
p53 is an important protein in the development of many cancers, acting as a tumour suppressor protein in many tumour types, inducing growth arrest or apoptosis depending on the physiological circumstances and cell type.
p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.
1. HOW DID OUR ANTIBODY PERFORM? ANTIBODY CUSTOMER REVIEW: p53 Polyclonal Antibody (STJ94890)
2. What was used? Primary antibody: p53 Antibody (STJ94890) Provider: St John’s Laboratory Dilution ratio: 1:500 Application: Western Blot Materials for validation: HepG2, HeLa, C6 and NIH3T3 cell lines Click for product data sheet PDF
3. What was the protocol? Primary Antibody Application 1:500 primary antibody was incubated at 4?C for overnight. Secondary Antibody Application Goat secondary antibody at 1:5000 for 1 hour at room temperature. Visualisation ECL kit. Exposure time 1 min.
4. What were the results?
5. JOIN THE ANTIBODY VALIDATION PROJECT
6. 1. Order any STJ9 catalogued product using this online form. 2. Test it in your laboratory using your preferred method. 3. Return a fully completed review form to receive rewards. ¡in 3 simple steps¡
7. @StJohnsLabs http://www.slideshare.net/stjohnslabs