Antibody Customer Review for Polyclonal Antibody (STJ92990) to cell signaling molecule ERK1/2


Target information

ERK 1/2 are kinases that play a crucial role in the MAP Kinase signal transduction pathway. This MAPK/ERK cascade plays a central role in many cellular processes including, but not limited to, cell survival, proliferation, cell growth, cellular adhesion and differentiation. ERK 1/2 are able to regulate these diverse processes through regulation of transcription, translation and cytoskeletal arrangement.

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1. HOW DID OUR ANTIBODY PERFORM? ANTIBODY CUSTOMER REVIEW: ERK1/2 Polyclonal Antibody (STJ92990)
2. What was used? Primary antibody: ERK 1/2 Antibody (STJ92990) Provider: St John’s Laboratory Dilution ratio: 1:500 Application: Western Blot Materials for validation: HepG2, HeLa, C6 and NIH3T3 cell lines Click for product data sheet PDF
3. What was the protocol? Primary Antibody Application 1:500 primary antibody was incubated at 4?C for overnight. Secondary Antibody Application Goat secondary antibody at 1:5000 for 1 hour at room temperature. Visualisation ECL kit. Exposure time 1 min.
4. What were the results?
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http://www.slideshare.net/stjohnslabs/antibody-customer-review-for-erk12-polyclonal-antibody-stj92990

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