Antibody Customer Review for SUMO1 Polyclonal Antibody (STJ95835)


Target information

SUMO1 (Small Ubiquitin-related Modifier 1) is a protein that functions in a similar manner to Ubiquitin, adding post-translational modifications to proteins. Unlike Ubiquitin however, which targets proteins for degradation, the modifications made by SUMO1 have a wide-range of functions in cellular processes, regulation nuclear transport, transcription, apoptosis and protein stability.

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1. HOW DID OUR ANTIBODY PERFORM? ANTIBODY CUSTOMER REVIEW: SUMO-1 Polyclonal Antibody (STJ95835)
2. What was used? Primary antibody: SUMO-1 Antibody (STJ95835) Provider: St John’s Laboratory Dilution ratio: 1:500 Application: Western Blot Materials for validation: HepG2, HeLa, C6 and NIH3T3 cell lines Click for product data sheet PDF
3. What was the protocol? Primary Antibody Application 1:500 primary antibody was incubated at 4?C for overnight. Secondary Antibody Application Goat secondary antibody at 1:5000 for 1 hour at room temperature. Visualisation ECL kit. Exposure time 1 min.
4. What were the results?
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Links

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