Customer Review For Cdc2 Polyclonal Antibody (STJ92154)


Target information

Cdc2 Polyclonal Antibody plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins.

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1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: Cdc2 Polyclonal Antibody (STJ92154)
2. WHAT WAS USED? Primary antibody: STJ92154. Cdc2 Polyclonal Antibody. 100ul Provider: St John’s Laboratory Dilution ratio: 1:1000 Application: Western Blot. Image: In HeLa and Jurkat cell lines, there were target bands detected by STJ92154 Cdc2 Polyclonal Antibody.
3. WHAT WAS THE PROTOCOL? Treatment of materials: When cells were cultured to 70%-80% plating density, lysis buffer with protease inhibitors (Sigma) and equal volume of 2X loading buffer were added for 10 min water bath at 100?C. Gel electrophoresis: 10% polyacrylamide gel, constant voltage 160 V, 60 min. Transfer: 0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60 min. Blocking: 1X TBST with 5% skimmed milk powder for 2 hr. Primary antibody probing: primary antibody was added to the solution of 1X TBST with 5% skimmed milk powder, 4?C for overnight. Membrane wash: 1X TBST wash for 3 times. Secondary antibody probing: secondary antibody was added to the solution of 1X TBST with 5% skimmed milk powder with 1£º10000 dilution ratio, for 1.5 hr. Membrane wash: 1X TBST wash for 3 times. Visualization: ECL visualization for 5 min (the visualization time was determined by actual result. It was ensured that the visualization time was identical for the same antigen).
4. WHAT WAS THE PROTOCOL? Gel electrophoresis information£º10% polyacrylamide gel, constant voltage 160 V, 60 min. Transfer information£º0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60 min. No. Antigen Loading amount Primary antibody Primary antibody dilution ratio Secondary antibody dilution ratio Target band KD Visualization time 1 HeLa lysate 10 ug STJ92154. Cdc2 Polyclonal Antibody 1:1000 1:10000 34KD 13S 2 Jurkat lysate 10 ug 1:10000 34KD 13S
5. WHAT DID THE CUSTOMER THINK? Testing results were provided by BPI Laboratory. They stated that ¡°In HeLa and Jurkat cell line, there were target bands detected by STJ92154 Cdc2 Polyclonal Antibody¡±.
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