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Recent Antibody Validation Data

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Organization/institution Yeshiva University
Validation data ID 131
Submission date 2017-03-01
Product name Rabbit Polyclonal Phospho-c-Abl (Y245) antibody (STJ90694)
Product type Primary antibody
Product catalog No. STJ90694
Supplier St John's Laboratory Ltd
Application type Western Blot
Antibody specificity rating Excellent
Antibody overall rating Excellent
Your comments

The antibody recognizes the correct Molecular size band. No other bands are obtained on Western blot.

Materials mouse spermatocyte lysate
Treatment of materials Non-denatirating lysis buffer
Gel electrophoresis NuPAGE 4 – 12% gradient Bis-Tris polyacrylamide gels and MOPS running buffer
Transfer proteins were transferred to a nitrocellulose membrane (0.45μm, Invitrogen, Carlsbad, CA, USA) using NuPAGE transfer buffer.
Blocking 2% membrane blocking agent (GE Healthcare UK Limited, RPN2125V, Little Chalfont, Buckinghamshire, UK) in PBS + 0.02% (v/v) Tween 20 (PBS-T)
Membrane wash PBS-T
Primary antibody incubation 1:500, RT, 2 hours
Secondary antibody
Name Supplier/brand Incubation
ECL™ anti-rabbit IgG HRP linked (GE Healthcare UK Limited, NA934V) 1Hour, RT
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1-3 spermatocytes 15-20 micrograms 1:500 1:5000 120kDa 1h
Result figure Result figure
Figure description

Abl is highly expressed in spermatocytes.

Product information
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