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Recent Antibody Validation Data

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Organization/institution The Evergreen State College
Validation data ID 189
Submission date 2017-05-12
Product name Rabbit Polyclonal Glucocorticoid receptor antibody
Product type Primary antibody
Product catalog No. STJ97101
Supplier St John's Laboratory Ltd
Application type Western Blot
Antibody specificity rating Good
Antibody overall rating Good
Your comments

Half sized band showed up as well, which has been previous reported by Expression and Distribution of Glucocorticoid Receptors in the
Ovine Fetal Adrenal Cortex: Effect of Long-term Hypoxia
Brandon Root, BA, Jenna Abrassart, BA, Dean A. Myers, PhD,
Tshepo Monau, MS, and Charles A. Ducsay, PhD

Materials Cow brain, 30ng samples
Treatment of materials Fresh, homogenized in RIPA with protease inhibitors
Gel electrophoresis 170mV for 50 min
Transfer 350mA for 60 min
Blocking 1 hour 5% nonfat milk in TBS
Membrane wash TBS-Tween (0.05%)
Primary antibody incubation overnight at degrees, 1:1000
Secondary antibody
Name Supplier/brand Incubation
Anti-rabbit IgG, HRP-linked Antibody Cell signalling 30 min room temperature, 1:3000
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 cow 30ng 1:1000 1:3000 ~97kDa 20 min
Result figure Result figure
Figure description

The blot that has a band is GR. The two samples are the same. Ponceau staining was done as well, but it will not let me upload this.

Product information
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