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Recent Antibody Validation Data

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Organization/institution The Evergreen State College
Validation data ID 190
Submission date 2017-05-12
Product name Rabbit polyclonal SOX2 antibody
Product type Primary antibody
Product catalog No. A0561
Supplier ABclonal
Application type Western Blot
Antibody specificity rating Not work in this case
Antibody overall rating Not work in this case
Your comments

Ponceau shows there is protein but Western did not work. These antibodies did sit at room temperature over the weekend upon arrival.

Materials cow brain, 30ug
Treatment of materials fresh, homogenized in RIPA and protease inhibitors
Gel electrophoresis 50 min 170 mV
Transfer 60min 350mA
Blocking 60 min 5% nonfat milk in TBS
Membrane wash TBS-Tween (0.05%)
Primary antibody incubation 1:1000
Secondary antibody
Name Supplier/brand Incubation
Anti-rabbit IgG, HRP-linked Antibody cell signaling 1:3000
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 cow brain 30ug 1:1000 1:3000 40 30min
Result figure Result figure
Figure description

membrane in the middle targets Sox2. A ponceau S shows there was protein but I cannot upload this.

Product information
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