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Recent Antibody Validation Data

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Organization/institution The Evergreen State College
Validation data ID 191
Submission date 2017-05-12
Product name Rabbit Polyclonal Estrogen Receptor alpha antibody
Product type Primary antibody
Product catalog No. STJ97499
Supplier St John's Laboratory Ltd
Application type Western Blot
Antibody specificity rating Not work in this case
Antibody overall rating Not work in this case
Your comments

ponceau s showed protein but Western did not work

Materials cow brain, 30ug
Treatment of materials fresh, homogenized in RIPA and protease inhibitors
Gel electrophoresis 50 min 170 mV
Transfer 60min 350mA
Blocking 60 min 5% nonfat milk in TBS
Membrane wash TBS-Tween (0.05%)
Primary antibody incubation 1:1000
Secondary antibody
Name Supplier/brand Incubation
Anti-rabbit IgG, HRP-linked Antibody cell signaling 1:3000
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 cow brain 30ug 1:1000 1:3000 ~70 30 min
Result figure Result figure
Figure description

membrane in the left targets ER. A ponceau S shows there was protein but I cannot upload this.

Product information
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