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Recent Antibody Validation Data

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Organization/institution Augusta University
Validation data ID 192
Submission date 2017-05-21
Product name anti-pan myristoylation antibody
Product type Primary antibody
Product catalog No. STJ98666
Supplier St John's Laboratory
Application type Western Blot
Antibody specificity rating Excellent
Antibody overall rating Excellent
Your comments

It is a very good antibody for western blotting

Materials Myc tagged gelsolin
Treatment of materials purified with anti-Myc beads
Gel electrophoresis SDS-PAGE 10%
Transfer 2 Hours
Blocking 5% milk
Membrane wash PBS+ 0.05%TWEEN 20
Primary antibody incubation 1:1000 dilution
Secondary antibody
Name Supplier/brand Incubation
HRP-Conjugated anti-rabbit sigma 1:5000, 1 hour at RT
Loading control antibody (optional)
Name Supplier/brand
lysate without Myc tagged gelsolin transfection no
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 purified Myc-gelsolin 10ul 1:1000 1:5000 80KD 1min
2 HEK293T cells without transfection but purified with anti-Myc beads 10ul 1:1000 1:5000 0 1min
Result figure Result figure
Figure description

we transfected myc-gelsolin into HEK293T cells and then purified it with anti-Myc beads, using non-transfected HEK293 lysates as control. We could detect a band about 80 KD in myc-gelsolin sample, but not control.

Product information
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