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Recent Antibody Validation Data

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Organization/institution augusta university
Validation data ID 193
Submission date 2017-05-21
Product name anti-P-cofilin
Product type Primary antibody
Product catalog No. STJ90230
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Excellent
Antibody overall rating Excellent
Your comments

It is a good antibody for western blotting

Materials HEK293T cells
Antibody incubation/dilution 1:20000
Experiment protocol

1, 15% SDS-PAGE gel to run samples
2, transfer to PVDF membrane
3, primary antibody, dilution with 1:20000, incubate overnight
4, wash with PBS +0.05% TWEEN 20 for four time
5, secondary antibody HRP conjugated anti-rabbit 1:5000 for 1 hour at RT

Result figure Result figure
Figure description

we used this antibody to detect p-cofilin in HEK293T cells and could detect a band around 18-19 KD.

Product information
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