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Recent Antibody Validation Data

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Organization/institution San Giovanni Rotondo
Validation data ID 195
Submission date 2017-05-26
Product name Rabbit Polyclonal FLAG-tag antibody
Product type Primary antibody
Product catalog No. STJ90108
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Fair
Antibody overall rating Good
Your comments

I used a dilution of 1:1000, incubating the membrane with the antibody overnight at 4 degrees.
I detected along with my specific protein band, also one unspecific which maybe adjusting the condtions I could get rid of it.

Materials 293T cell transfected. cell lysed with NP-40 lysis buffer
Treatment of materials boiled with laemmli buffer at 90 degrees for 10 minutes
Gel electrophoresis 7%
Transfer transblot turbo biorad, 30 minutes
Blocking TBS, milk 5%
Membrane wash TBS-T 0.3%
Primary antibody incubation overnight
Secondary antibody
Name Supplier/brand Incubation
Goat Anti-Rabbit IgG Antibody GenScript 1 hour at room temperature
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
15ug 1:1000 1:10000 80 30 seconds
Result figure antibody-validation-FlagTag.pdf
Figure description

The figure shows a protein of 80KDa.
The acquisition was performed using an X-Ray film, the specific band is clearly visible but it would be excellent without the non spefific band.

Product information
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