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Recent Antibody Validation Data
|Share with your collegues|
|Organization/institution||San Giovanni Rotondo|
|Validation data ID||195|
|Product name||Rabbit Polyclonal FLAG-tag antibody|
|Product type||Primary antibody|
|Product catalog No.||STJ90108|
|Supplier||St John's Laboratory|
|Application type||Western Blot(WB)|
|Antibody specificity rating||Fair|
|Antibody overall rating||Good|
I used a dilution of 1:1000, incubating the membrane with the antibody overnight at 4 degrees.
|Materials||293T cell transfected. cell lysed with NP-40 lysis buffer|
|Treatment of materials||boiled with laemmli buffer at 90 degrees for 10 minutes|
|Transfer||transblot turbo biorad, 30 minutes|
|Blocking||TBS, milk 5%|
|Membrane wash||TBS-T 0.3%|
|Primary antibody incubation||overnight|
|Sample Loading Information||
The figure shows a protein of 80KDa.
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