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Recent Antibody Validation Data

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Organization/institution ISBReMIT
Validation data ID 196
Submission date 2017-05-26
Product name c-Myc Tag Antibody, mAb, Mouse
Product type Primary antibody
Product catalog No. A00704-100
Supplier GenScript
Application type Western Blot(WB)
Antibody specificity rating Excellent
Antibody overall rating Excellent
Your comments

It worked great

Materials 293T cell transfected. cell lysed with NP-40 lysis buffer
Treatment of materials Sample was boiled with laemmli buffer at 90 degrees for 10 minutes
Gel electrophoresis 7% polyacrylamide
Transfer transblot turbo biorad, 30 minutes
Blocking TBS, milk 5%
Membrane wash TBS-T 0.3%
Primary antibody incubation overnight
Secondary antibody
Name Supplier/brand Incubation
Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb GenScript 1 hour at room temperature
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
15ug 1:1000 1:10000 94 30 seconds
Result figure antibody-validation-Myc-Tag.pdf
Figure description

The figure show a protein of 94KDa,
The expure time using X-Ray film was for 30 seconds

Product information
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