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Recent Antibody Validation Data

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Organization/institution Winship Cancer Institute
Validation data ID 201
Submission date 2017-06-29
Product name Rabbit Polyclonal Phospho-FoxO3A (S253) antibody
Product type Primary antibody
Product catalog No. STJ90275
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Good
Your comments

This antibody worked well for me. I had tried antibodies from other companies also but only this worked.

Materials Human cell lines
Treatment of materials No treatment
Gel electrophoresis 200V , 30min
Transfer TG buffer+methanol
Blocking 1hr in milk
Membrane wash 5min three times
Primary antibody incubation overnight
Secondary antibody
Name Supplier/brand Incubation
Rabbit cell signaling 1hr RT
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 KMS11 20ug 1:1000 1:5000 97KD 45seconds
Result figure p-Foxo3a.pdf
Figure description

Human multiple myeloma cell line KMS11 was cultured in RPMI-8226 media and then lysates are made in RIPA buffer supplemented with phosphatase and proteinase inhibitors. 2ug of protein was loaded in two wells and electrophoresed for 30min at 200V BIORAD electrophoresis unit. Gel was transferred to PVDF membrane and blot evaluated for the expression of p-FOXO3a.

Product information
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