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Recent Antibody Validation Data
|Share with your collegues|
|Organization/institution||Winship Cancer Institute|
|Validation data ID||201|
|Product name||Rabbit Polyclonal Phospho-FoxO3A (S253) antibody|
|Product type||Primary antibody|
|Product catalog No.||STJ90275|
|Supplier||St John's Laboratory|
|Application type||Western Blot(WB)|
|Antibody specificity rating||Good|
|Antibody overall rating||Good|
This antibody worked well for me. I had tried antibodies from other companies also but only this worked.
|Materials||Human cell lines|
|Treatment of materials||No treatment|
|Gel electrophoresis||200V , 30min|
|Blocking||1hr in milk|
|Membrane wash||5min three times|
|Primary antibody incubation||overnight|
|Sample Loading Information||
Human multiple myeloma cell line KMS11 was cultured in RPMI-8226 media and then lysates are made in RIPA buffer supplemented with phosphatase and proteinase inhibitors. 2ug of protein was loaded in two wells and electrophoresed for 30min at 200V BIORAD electrophoresis unit. Gel was transferred to PVDF membrane and blot evaluated for the expression of p-FOXO3a.
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