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Recent Antibody Validation Data
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|Validation data ID||203|
|Product name||Anti-Connexin 40 antibody|
|Product type||Primary antibody|
|Product catalog No.||STJ96742|
|Supplier||St John's Laboratory|
|Application type||Immunocytochemistry (ICC)|
|Antibody specificity rating||Not work in this case|
|Antibody overall rating||Not work in this case|
The antibody had some non-specific binding in the negative control. The experimental did not show clear dots which is what is expected for connexin binding.
The antibody was tested for functionality in cell immunofluorescence. The sample was C2C12 mouse muscle cells transfected with a plasmid that expresses connexin 40 IRES FusionRed under a mature muscle promoter MCK. The negative control were the same cells with just the FusionRed tag. It appears that there is light non-specific binding.
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