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Recent Antibody Validation Data

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Organization/institution Tufts University
Validation data ID 203
Submission date 2017-06-30
Product name Anti-Connexin 40 antibody
Product type Primary antibody
Product catalog No. STJ96742
Supplier St John's Laboratory
Application type Immunocytochemistry (ICC)
Antibody specificity rating Not work in this case
Antibody overall rating Not work in this case
Your comments

The antibody had some non-specific binding in the negative control. The experimental did not show clear dots which is what is expected for connexin binding.

Result figure
  • Result figure
  • Result figure
Figure description

The antibody was tested for functionality in cell immunofluorescence. The sample was C2C12 mouse muscle cells transfected with a plasmid that expresses connexin 40 IRES FusionRed under a mature muscle promoter MCK. The negative control were the same cells with just the FusionRed tag. It appears that there is light non-specific binding.

Product information
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