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Recent Antibody Validation Data
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|Validation data ID||204|
|Product name||Anti-Connexin 43 antibody|
|Product type||Primary antibody|
|Product catalog No.||STJ92411|
|Supplier||St John's Laboratory|
|Application type||Immunocytochemistry (ICC)|
|Antibody specificity rating||Excellent|
|Antibody overall rating||Excellent|
This antibody worked very well for this assay.
C2C12 mouse muscle cells were transfected with a plasmid expressing connexin 43 IRES FusionRed under the control of a mature muscle promoter MCK. Normally C2C12 cells do not express Connexin 43 in mature myotubes. They do express connexin 43 in immature myoblasts. The negative control are the same cells with the FusionRed tag only. There is still some staining of connexin 43, but not in the mature myotubes.
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