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Recent Antibody Validation Data

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Organization/institution Boston Children's Hospital
Validation data ID 206
Submission date 2017-06-30
Product name Rabbit polyclonal GAS6 antibody (A8545)
Product type Primary antibody
Product catalog No. A8545
Supplier ABclonal
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Good
Your comments

This lot of Gas6 antibody works well on my WB, hopefully, you have enough antibody for our research from the same lot.

Materials mouse brain tissue and secreted protein in culture medium
Treatment of materials denature with SDS loading buffer
Gel electrophoresis SDS seperate gel
Transfer regular transfer buffer with 20% methonal
Blocking 5% non-fat milk in TBS-T buffer
Membrane wash TBS-T buffer
Primary antibody incubation in 5% non-fat milk in TBS-T buffer
Secondary antibody
Name Supplier/brand Incubation
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody A6154 Sigma in 5% non-fat milk in TBS-T buffer
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 WT mouse brain tissue 10ug total protein 1:1500 1:4000 70-80KDa 10minutes
2 Gas6 KO mouse brain tissue 10ug total protein 1:1500 1:4000 no band 10minutes
3 culture medium from Gas6 transfected 293T cell 10ul 1:1500 1:4000 70-80KDa 10minutes
4 culture medium from mock transfected 293T cell 10ul 1:1500 1:4000 no band 10minutes
Result figure Result figure
Figure description

Figure is labelled with sample names very well

Product information
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