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Recent Antibody Validation Data

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Organization/institution UT Southwestern Medical Center
Validation data ID 208
Submission date 2017-07-17
Product name Rabbit Polyclonal Neu Antibody
Product type Primary antibody
Product catalog No. STJ94416
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Good
Your comments


Materials Heart extract from neonatal mice
Treatment of materials No
Gel electrophoresis 90 V/ 3 hrs
Transfer Turbo transfer
Blocking 5% skim milk/ 1 hrs
Membrane wash 0.05% TBST
Primary antibody incubation 1:500 dilution in 0.05% TBST, over night at 4 degree
Secondary antibody
Name Supplier/brand Incubation
IRDye® 800CW Secondary Antibodies (Rabbit) Licor 1 hrs at room temperature
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 Heart extract (neonatal mouse) 10 ug 1:500 1:10000 180-200 kD 2 min
Result figure Result figure
Figure description

A clear band of Her2 has been detected.

Product information
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