Why join AntibodyPlus Review Project?

1. Publishing a paper takes 1-2 years while a single batch of antibody product will only be available for 3-6 months. Actually, you are not able to get the exactly same antibody in the citation. Up-to-date reviews reflects antibodies quality better in the long term. Learn more about validation

2. Researchers participate, share reviews (regardless of positive or negative) and get at least $50 Abcoins in return. Check more rewards

3. Our scientists from will consult the results with you and troubleshoot any issues in your experiments. If your result is negative, we offer alternative solutions and replacements. See our promise

Recent Antibody Validation Data

Share with your collegues
Organization/institution Louisiana State University
Validation data ID 226
Submission date 2017-09-01
Product name Anti-ACAP1
Product type Primary antibody
Product catalog No. CSBPA622985EA01 HU
Supplier Cusabio
Application type Western Blot(WB)
Antibody specificity rating Average
Antibody overall rating Good
Your comments

Saw two strong bands near 75-85kDa. no other obvious background

Materials crude extract proteins from A375 melanoma cells
Treatment of materials none
Gel electrophoresis SDS-PAGE
Transfer PVDF membrane
Blocking 5% skim milk/TBST
Membrane wash TBST/ 15min each wash/ 3X total
Primary antibody incubation 1000X dilution/ overnight/ 4C
Secondary antibody
Name Supplier/brand Incubation
Anti-rabbit HRP pierce 3 hours at room temp
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 ACAP1-melanoma 10ug 1000X 2000X 85kD 1 second
Result figure Result figure
Figure description

top band is near 85kDa and should be the ACAP1. bottom band is probably non-specific.

Product information
Free Sample Project

We invite you to join our Free Sample Project!