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Recent Antibody Validation Data

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Organization/institution Emory University
Validation data ID 227
Submission date 2017-09-06
Product name Anti-Claudin-2 antibody
Product type Primary antibody
Product catalog No. STJ92312
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Good
Your comments

We had good success on multiple samples using this antibody.

Materials Gut lysate
Treatment of materials Protein extraction
Gel electrophoresis Yes
Transfer Yes
Blocking Yes
Membrane wash Yes
Primary antibody incubation Overnight 4 degree
Secondary antibody
Name Supplier/brand Incubation
Goat anti-rabbit HRP linked Invitrogen
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
2 WT 20ug 1:1000 1:2000 25 5 min
Result figure Result figure
Figure description

Lane 2 - WT B6 mice gut with CLDN 2 detected
Lane 3 - Septic mouse with decreased CLDN 2

Product information
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