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Recent Antibody Validation Data

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Organization/institution Emory University
Validation data ID 228
Submission date 2017-09-06
Product name CLDN1 Polyclonal Antibody
Product type Primary antibody
Product catalog No. A2196
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Not work in this case
Antibody overall rating Not work in this case
Your comments

Unfortunately this antibody did not work.

Materials Mouse Gut lysate
Treatment of materials Protein extraction
Gel electrophoresis Yes
Transfer Yes
Blocking Yes
Membrane wash Yes
Primary antibody incubation Overnight 4 degree
Secondary antibody
Name Supplier/brand Incubation
Goat anti-rabbit HRP linked Invitrogen Per protocol
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 Ladder 5 ul
2 WT B6 Gu 20 ug 1:1000 1:2000 22 SAM
Result figure Result figure
Figure description

Lanes 2-13 are varying in time points of sepsis - no observed band at target site

Product information
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