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Recent Antibody Validation Data

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Organization/institution Emory University
Validation data ID 229
Submission date 2017-09-06
Product name OCLN Polyclonal Antibody
Product type Primary antibody
Product catalog No. A2601
Supplier ABclonal
Application type Western Blot(WB)
Antibody specificity rating Not work in this case
Antibody overall rating Not work in this case
Your comments

This is the second ABclonal Ab I have tried, both of which did not work.

Materials Mouse Gut lysate
Treatment of materials Protein extraction
Gel electrophoresis yes
Transfer yes
Blocking yes
Membrane wash yes
Primary antibody incubation overnight 4 degree
Secondary antibody
Name Supplier/brand Incubation
Goat anti-rabbit HRP linked Goat anti-rabbit HRP linked Per protocol
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 Ladder 5 ul
2 WT B6 gut lysate 40 ug 1:1000 1:2000 59 SAM
Result figure Result figure
Figure description

1. Too many nonspecific bands despite blocking for 1.5 hrs (typical protocol is 1 hour)
2. Lanes 6 and 7 have a band that could be 59 KD, however our WT sample should have OCLDN expression as we have demonstrated in previous experiments and in RT PCR

Product information
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