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Recent Antibody Validation Data

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Organization/institution Emory University
Validation data ID 231
Submission date 2017-09-07
Product name Anti-Jam-A antibody
Product type Primary antibody
Product catalog No. STJ96844
Supplier St John's Laboratory
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Fair
Your comments

Unclear - we have some bands near in btw 50 and 37 kD (JAM is 32), we are unsure that this worked

Materials Mouse Gut lysate
Treatment of materials Protein extraction
Gel electrophoresis yes
Transfer yes
Blocking yes
Membrane wash yes
Primary antibody incubation Overnight 4 degree
Secondary antibody
Name Supplier/brand Incubation
Goat anti-rabbit HRP linked Invitrogen Per protocol
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 ladder
2 wt
4 Septic mice 20 ug 1:1000 1:2000 32 SAM
Result figure Result figure
Figure description

JAM-A should up regulate during septic insult. Lanes 2 and 3 are WT while the rest are septic mice. We have bands but unclear if they are our target.

Product information
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