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Recent Antibody Validation Data

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Organization/institution McGill University
Validation data ID 249
Submission date 2017-10-23
Product name Rabbit polyclonal RPS3 antibody (A2533)
Product type Primary antibody
Product catalog No. A2533
Supplier ABclonal
Application type Western Blot(WB)
Antibody specificity rating Excellent
Antibody overall rating Excellent
Your comments

Expected size around 27kDa; size observed 30kDa.

Materials Ba/F3 cell line
Treatment of materials Wild-type protein extracted for Western blot
Gel electrophoresis 1hr 65V, 1hr 150V in running buffer
Transfer 1hr, 100V in transfer buffer with methanol, on to nitrocellulose membrane
Blocking 1hr in 5% milk in TBS-T
Membrane wash TBS-T
Primary antibody incubation Overnight
Secondary antibody
Name Supplier/brand Incubation
Goat Anti-Rabbit IgG Abcam 1 hour
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
2 WT 25ug 1:1000 1:10000 30kDa 3 seconds
Result figure Result figure
Figure description

Validation of Rps3 antibody specificity in Ba/F3 cells.

Product information
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