Why join AntibodyPlus Review Project?

1. Publishing a paper takes 1-2 years while a single batch of antibody product will only be available for 3-6 months. Actually, you are not able to get the exactly same antibody in the citation. Up-to-date reviews reflects antibodies quality better in the long term. Learn more about validation

2. Researchers participate, share reviews (regardless of positive or negative) and get at least $50 Abcoins in return. Check more rewards

3. Our scientists from will consult the results with you and troubleshoot any issues in your experiments. If your result is negative, we offer alternative solutions and replacements. See our promise

Recent Antibody Validation Data

Share with your collegues
Organization/institution Karolinska Institutet
Validation data ID 253
Submission date 2017-11-30
Product name Rabbit polyclonal TARBP2 antibody
Product type Primary antibody
Product catalog No. A7533
Supplier ABclonal
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Good
Your comments

Despite we noted multiple bands, the expected band was easily distinct from non-specific bands.

Materials Human cell lines
Treatment of materials sicontrol and two different siRNAs targeting TARBP2
Gel electrophoresis 4-12% SDS PAGE
Transfer NOVEX
Blocking 5% milk
Membrane wash TBST
Primary antibody incubation 4 oC overnight
Secondary antibody
Name Supplier/brand Incubation
Anti-rabbit IgG HRP Cell Signaling 1 hour at room temperature
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
2 HEK293T 30 ug 1:1000 1:2000 46 5s
3 HEK293T 30ug 1:1000 1:2000 46 5s
4 HEK293T 30ug 1:1000 1:2000 46 5s
Result figure abclonal-TARBP2-antibody-review-image.pdf
Figure description

The figure shows HEK293T cells transfected with sicontrol (siCTRL, lane 2) and two different siRNAs targeting to TARBP2 (siTARBP2-1 and siTARBP2-2; lane 3 and 4)

Product information
Free Sample Project

We invite you to join our Free Sample Project!