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Recent Antibody Validation Data

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Organization/institution Baylor College of Medicine
Validation data ID 293
Submission date 2019-09-09
Product name Rabbit polyclonal GATA2 antibody
Product type Primary antibody
Product catalog No. A0677
Supplier ABclonal
Application type Western Blot(WB)
Antibody specificity rating Good
Antibody overall rating Good
Your comments

Houston

Materials CELL
Treatment of materials siRNA transfection
Gel electrophoresis BIO-RAD, 456-1086, 4-15%
Transfer 300mA, 1HR
Blocking 5% non-fat milk, 0.5hr, RT
Membrane wash TBST, 3X10 min, RT
Primary antibody incubation 4℃, overnight
Secondary antibody
Name Supplier/brand Incubation
goat anti Rabbit cell signaling RT, 1hr
Loading control antibody (optional)
Name Supplier/brand
actin santa cruz
Sample Loading Information
Lane No. Sample Loading amount Primary Antibody dilution Secondary Antibody dilution Target band (KD) Exposure Time
1 22RV1-siCTRL 20ug 1000 2000 46 1 min
2 22RV1-siGATA2 20ug 1000 2000 46 1min
3 LNCaP-siCTRL 20ug 1000 2000 46 1min
4 LNCaP-siGATA2 20ug 1000 2000 46 1min
Result figure Result figure
Figure description

22RV-1 and LNCaP cells were transfected with siRNA for 3 days, cells were lysed in RIPA buffer and western blot as described.

Product information
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