HMGB1 Review: JAK / STAT pathway mediates the expression of high mobility group boxer B1 mRNA in liver tissue of rats with sepsis

HMGB1 review and articles

OBJECTIVE: To investigate the expression of high mobility group box-1 (HMGB1) mRNA in liver tissue of rats with sepsis induced by Janus kinase / signal transduction and transcription activator (JAK / STAT) pathway in patients with cecal ligation (CLP) Acute liver damage. Methods: The rats were randomly divided into normal control group, CL P sepsis group, JAK2 kinase inhibitor AG490 and STAT inhibitor rapamycin (RPM) treated group. The liver HMGB1 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR), and the liver function was measured by automatic biochemical analyzer. Results: Compared with the normal control group, the expression of HMGB1 mRNA was significantly increased at 6 ~ 48h after CL P (P <0.01). Serum aspartate aminotransferase (AST) increased significantly at 6 ~ 48h (P <0.01). 0), alanine aminotransferase (AL T) and AST were significantly increased at 24 h (P <0.01). Compared with CL P group, the levels of HMGB1m RNA and ALT were significantly decreased in AG490 pretreatment group (P <0.01), and the AST was significantly lower at 24 h and 48 h (P <0.01) The expression of HMGB1m RNA was significantly inhibited at 6 h and 24 h after RPM intervention (P <0.05 and P <0.01). ALT and AST decreased at 24 h and 48 h (P <0.01) 1 and P <0 05). Conclusion: Inhibition of JAK / STAT pathway activation can downregulate the expression of HMGB1 mRNA in liver tissue and contribute to the alleviation of acute liver injury induced by CL P.

Reference:
Wang SB, Yao YM, Dong N, Yu Y, Tao GC. [Role of Janus kinase/signal transducer and activator of transcription pathway in mediating mRNA expression of high mobility group box1 protein in the liver in septic rats]. Zhongguo Wei Zhong Bing
Ji Jiu Yi Xue. 2003 Mar;15(3):147-9. Chinese. PubMed PMID: 12831616.
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