25-OH Vitamin D ELISA Kit (ARG80775)

$749.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity
Tested Application ELISA
Specificity
Target Name 25-OH Vitamin D
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 2.81 ng/ml
Detection Range
Sample Type Serum and plasma
Sample Volume 50 ul
Precision

CV: <10%

Full Name 25-OH Vitamin D
Alternate Names Check alternative names for the 25-OH Vitamin D ELISA Kit

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Application Instructions

 

Assay Time 2 h, 30, 15 min (RT/shaker)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of 25-OH Vitamin D ELISA Kit  Download
size

96 wells

Cat No.

ARG80775

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

2.81 ng/ml

Sample type

Serum and plasma

Sample Volume

50 ul

Precision

CV: <10%

Target name

25-OH Vitamin D

Assay time

2 h, 30, 15 min (RT/shaker)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Vitamin D is the generic term used to designate Vitamin D2 or ergocalciferol and Vitamin D3 or cholecalciferol. Humans naturally produce Vitamin D3 when the skin is exposed to ultraviolet sun rays. In the liver mainly, Vitamin D3 is metabolised into 25-Hydroxyvitamin D3 (25-OH D3) which is the main form of Vitamin D circulating in the body. 25OH D3 is a precursor for other Vitamin D metabolites and has also a limited activity by itself. The most active derivative is 1,25-hydroxyvitamin D3, produced in the kidney (or placenta) by 1?hydroxylation of 25-OH D3. 25-OH Vitamin D stimulates the intestinal absorption of both calcium and phosphorus and also bone resorption and mineralisation. 25-OH Vitamin D might also be active in other tissues responsible for calcium transport (placenta, kidney, mammary gland ¡­) and endocrine gland (parathyroid glands, beta cells¡­). Vitamin D3 and Vitamin D2 are also available by ingestion through food or dietary supplementation. As Vitamin D2 is metabolised in a similar way to Vitamin D3, both contribute to the overall Vitamin D status of an individual. It is the reason why it is very important to measure both forms of 25-OH Vitamin D equally for a correct diagnosis of Vitamin D deficiency, insufficiency or intoxication. Vitamin D deficiency is an important risk factor for rickets, osteomalacia, senile osteoporisis, cancer and pregnancy outcomes. The measurement of both 25-OH Vitamin D forms is also required to determine the cause of abnormal serum calcium concentrations in patients. Vitamin D intoxication has been shown to cause kidney and tissue damages.

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