8-OHdG ELISA Kit (ARG80857)

$1,099.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Other
Tested Application ELISA
Specificity
Target Name 8-OHdG
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.5 ng/ml
Detection Range
Sample Type Saliva
Sample Volume 50 ul
Precision

CV: <10%

Full Name 8-Hydroxy-2′-Deoxyguanosin
Alternate Names Check alternative names for the 8-OHdG ELISA Kit

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Application Instructions

 

Assay Time 2 x 1 h (37 Celcius degree), 15 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of 8-OHdG ELISA Kit  Download
size

96 wells

Cat No.

ARG80857

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.5 ng/ml

Sample type

Saliva

Sample Volume

50 ul

Precision

CV: <10%

Target name

8-OHdG

Assay time

2 x 1 h (37 Celcius degree), 15 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

The assay system is a competitive binding assay using plates which are pre-coated with 8-OHdG. The monoclonal antibody is supplied in liquid form. Therefore higher concentrations of 8-OHdG will result in a lower signal at the end of the assay procedure. Salivary 8-OHdG seems to be a useful marker for oxidative damage of DNA or oxydative stress in total. Oxidative stress is a major cause of endothelial dysfunction. Oxidative stress produces reactive oxygen species, mainly superoxide, and may promote endothelial dysfunction by inactivating the production of NO. Oxidative stress might play a significant role in the pathophysiologic mechanism of erectile dysfunction. There is some evidence that the measurement of salivary 8OHdG also is a useful biomarker for predicting severe erectile dysfunction and hypogonadism. [Yasuda et al., 2008. Journal of Sexual Medicine]

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