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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Volume||50 ul|
|Alternate Names||Check alternative names for the 8-OHdG ELISA Kit Expand|
|Assay Time||2 x 1 h (37 Celcius degree), 15 min (RT)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of 8-OHdG ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
|Sample Volume|| |
|Target name|| |
|Assay time|| |
2 x 1 h (37 Celcius degree), 15 min (RT)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
The assay system is a competitive binding assay using plates which are pre-coated with 8-OHdG. The monoclonal antibody is supplied in liquid form. Therefore higher concentrations of 8-OHdG will result in a lower signal at the end of the assay procedure. Salivary 8-OHdG seems to be a useful marker for oxidative damage of DNA or oxydative stress in total. Oxidative stress is a major cause of endothelial dysfunction. Oxidative stress produces reactive oxygen species, mainly superoxide, and may promote endothelial dysfunction by inactivating the production of NO. Oxidative stress might play a significant role in the pathophysiologic mechanism of erectile dysfunction. There is some evidence that the measurement of salivary 8OHdG also is a useful biomarker for predicting severe erectile dysfunction and hypogonadism. [Yasuda et al., 2008. Journal of Sexual Medicine]
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