Acetyl-Histone H3-K14 Polyclonal Antibody-ChIP Grade (A7254)

$259.00

Reactivity: Human,Mouse,Rat, Wide range
Applications: WB, IHC, IF, IP, ChIP, ChIPseq
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Background


Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information


Immunogen A synthetic acetylated peptide corresponding to residues surrounding K14 of human H3
Gene ID 8290
Swiss Prot Q16695
Synonyms H3.4;H3/g;H3FT;H3t
Calculated MW 16kDa
Observed MW Refer to Figures

Applications


Reactivity Human Mouse Rat Other (Wide Range)
Tested applications WB IHC IF IP ChIP ChIPseq
Recommended Dilution WB 1:1000 – 1:3000
IHC 1:200 – 1:500
IF 1:500 – 1:1000
IP 1:200 – 1:500
CHIP 1:50 – 1:100
CHIPseq 1:50 – 1:100
Calculated MW 16kDa
Observed MW Refer to Figures
Immunogen A synthetic acetylated peptide corresponding to residues surrounding K14 of human H3
Storage Buffer Store at -20 Celcius Degree. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonym H3.4;H3/g;H3FT;H3t
Positive samples BT-474,293T,H3 protein
Cellular Location Nucleus,Chromosome

Other information


Storage instructions Store at -20 Celcius Degree. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Reactivity

Human, Mouse, Rat, Wide range

Clonality

polyclonal

Applications

WB, IHC, IF, IP, ChIP, ChIPseq

Supplier

ABclonal Inc.

Product type

Primary antibody

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