Aflatoxin B1 ELISA Kit (ARG80786)

$699.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity
Tested Application ELISA
Specificity
Target Name Aflatoxin B1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 5 pg/ml
Detection Range
Sample Type food (extraction, dilution)
Sample Volume 100 ul
Precision

CV: <10%

Full Name Aflatoxin B1
Alternate Names Check alternative names for the Aflatoxin B1 ELISA Kit

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Application Instructions

 

Assay Time 1 h, 1 h (RT/shaker), 20 min (RT/dark)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Aflatoxin B1 ELISA Kit  Download
size

96 wells

Cat No.

ARG80786

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

5 pg/ml

Sample type

food (extraction, dilution)

Sample Volume

100 ul

Precision

CV: <10%

Target name

Aflatoxin B1

Assay time

1 h, 1 h (RT/shaker), 20 min (RT/dark)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Aflatoxins belong to the class of mycotoxins. Che?mic?ally they are defined as difuranocyclopenta?no?cu?marines or difuranopentanolidocu?marines, i.e. afla?toxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is con?densed. Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The deri?vates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions. Aflatoxins belong to the strongest mycotoxins, which act primarily in a hepatotoxic and carcinogenic way. The four main aflatoxins show a different toxicity. B1 is without doubt the most toxic aflatoxin, followed by G1, B2 and G2. Aflatoxin B1, however, does not show a direct toxic action. In the process of biotransformation in the liver, the lipophilic toxin is epoxidated and transformed into an active derivative, the so-called aflatoxin B1-2,3-epoxid. This highly reactive epoxid is able to react with nucleophilic regions of macromolecules. Amongst other this metabolite of aflatoxin B1 binds covalently to the N-7 atom of the guanine bases of DNA. This covalent bond causes an inhibition of the DNA replication, the RNA synthesis and mutations. Both chronical and acute intoxications are ef?fected by aflatoxins. There are only few documented reports about acute intoxications, which are caused by uptake of mycotoxins. Of special importance for hu?man beings are the chronical intoxications by aflatoxins. To the diseases, which develop after such chronical intoxications, belong primary liver carcinoma, hepatitis, Reye?s syndrome and Kwashiorkor. Besides the generation of primary liver carcinoma, aflatoxins are presumably also responsible for other sorts of tumors, like intestinal cancer. Contaminations with aflatoxins occur mostly with nuts and grain. In most cases aflatoxins penetrate the human body via the food. Aflatoxins are stable to heat and are only partly destroyed by boiling. In order to protect people against aflatoxin-induced diseases, there is a need for the quantitative and qualitative control of endangered foodstuff, besides appropriate hygienic precautions, which avoid the formation of aflatoxins. The Aflatoxin B1 ELISA is a quick, economical and sensitive method to detect aflatoxin B1 in food. After an appropriate sample preparation, 40 samples can be tested in du?plicate within 140 minutes.

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