Anti-EGLN1 Antibody

$99.00$319.00

Reactivity: Human,Rat
Applications: ELISA,WB,IHC
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-EGLN1 Antibody (CSB-PA863932ESR2HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

Hypoxia-inducible factor prolyl hydroxylase 2 Antibody, HIF-PH2 Antibody,HIF-prolyl hydroxylase 2 Antibody,HPH-2 Antibody,Prolyl hydroxylase domain-containing protein 2 Antibody,PHD2 Antibody,SM-20 Antibody,EGLN1 Antibody

More alternative names for the antibody
C1ORF12 antibody|Chromosome 1 Open Reading Frame 12 antibody|DKFZp761F179 antibody|ECYT 3 antibody|ECYT3 antibody|EGL 9 homolog of C. elegans 1 antibody|EGL nine (C.elegans) homolog 1 antibody|Egl nine homolog 1 (C. elegans) antibody|Egl nine homolog 1 antibody|Egl nine like protein 1 antibody|EGLN 1 antibody|Egln1 antibody|EGLN1_HUMAN antibody|HIF PH2 antibody|HIF Prolyl Hydroxylase 2 antibody|HIF-PH2 antibody|HIF-prolyl hydroxylase 2 antibody|HIFP4H 2 antibody|HIFPH2 antibody|HPH 2 antibody|HPH-2 antibody|HPH2 antibody|Hypoxia inducible factor prolyl hydroxylase 2 antibody|Hypoxia-inducible factor prolyl hydroxylase 2 antibody|ORF13 antibody|P4H2 antibody|PHD 2 antibody|PhD2 antibody|PNAS 118 antibody|PNAS 137 antibody|Prolyl Hydroxylase Domain Containing Protein 2 antibody|Prolyl hydroxylase domain-containing protein 2 antibody|Rat Homolog of SM20 antibody|SM 20 antibody|SM-20 antibody|SM20 antibody|Zinc finger MYND domain containing protein 6 antibody|ZMYND6 antibody
Anti-PHD2 / prolyl hydroxylase antibody (ab4561)
Close sc-34920|sc-34921|sc-67030|sc-271835|sc-34923|

Recommended applications: ELISA, WB, IHC

Recommended dilution: Recommended dilution:WB:1:500-2000,IHC:1:20-1:200

Recommended protocols: check protocols

Name

Anti-EGLN1 Antibody

Catalogue No.

CSB-PA863932ESR2HU

Reactivity

Human, Rat

Immunogen

Recombinant human Egl nine homolog 1 protein (301-426AA)

Host

Rabbit

Applications

ELISA, WB, IHC

Recommended dilution

Recommended dilution:WB:1:500-2000,IHC:1:20-1:200

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

46 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:54583( Human), Omim:606425( Human), SwissProt:Q9GZT9( Human), Unigene:444450( Human)

Protein function

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif. .

Protein tissue specificity

According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle. .

Involvement in disease

Erythrocytosis, familial, 3 (ECYT3) [MIM:609820]: An autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels. . Note=The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The beta(2)beta(3) ‘finger-like’ loop domain is important for substrate (HIFs’ CODD/NODD) selectivity.

Protein post-translational modifications

S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-323 or Cys-326. .

Protein cellular localization

Cytoplasm . Nucleus . Note=Mainly cytoplasmic. Shuttles between the nucleus and cytoplasm (PubMed:19631610). Nuclear export requires functional XPO1. .

Research area

All research areas>Signaling Intermediates>Prolyl Hydroxylase
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus provides antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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