Anti-GOLGA2 Antibody

$99.00$319.00

Reactivity: Human
Applications: ELISA,IHC
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-GOLGA2 Antibody (CSB-PA600856ESR1HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

130 kDa cis-Golgi matrix protein Antibody,GM130 Antibody,GM130 autoantigen Antibody,Golgin-95 Antibody,GOLGA2 Antibody

More alternative names for the antibody
130 kDa cis Golgi matrix protein antibody|130 kDa cis-Golgi matrix protein antibody|Cis golgi matrix protein GM130 antibody|GM130 antibody|Gm130 autoantigen antibody|GOGA2_HUMAN antibody|GOLGA 2 antibody|Golga2 antibody|Golgi autoantigen antibody|Golgi autoantigen golgin subfamily a 2 antibody|Golgi matrix protein GM130 antibody|Golgin 95 antibody|golgin A2 antibody|Golgin subfamily a 2 antibody|Golgin subfamily A member 2 antibody|Golgin-95 antibody|MGC20672 antibody|SY11 protein antibody
Anti-GM130 antibody [EP892Y] – cis-Golgi Marker (ab52649)
Close sc-55591|sc-31148|sc-30100|sc-55590|sc-16271|sc-16268|sc-16270|

Recommended applications: ELISA, IHC

Recommended dilution: Recommended dilution:IHC:1:20-1:200

Recommended protocols: check protocols

Name

Anti-GOLGA2 Antibody

Catalogue No.

CSB-PA600856ESR1HU

Reactivity

Human

Immunogen

Recombinant human Golgin subfamily A member 2 protein (20-300AA)

Host

Rabbit

Applications

ELISA, IHC

Recommended dilution

Recommended dilution:IHC:1:20-1:200

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

113 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:2801( Human), Omim:602580( Human), SwissProt:Q08379( Human), Unigene:155827( Human)

Protein function

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401). .

Protein sequence and domain

Belongs to the GOLGA2 family. The nuclear localization signal (cNLS) mediates interaction with importin-alpha, recruiting importin-alpha to the Golgi membrane and liberating TPX2.

Protein post-translational modifications

Cleaved by caspases at the onset of apoptosis. .; Methylation by PRMT5 is required for Golgi ribbon formation. While dimethylation at Arg-30 and Arg-35 are confirmed in vivo, it is unclear whether Arg-18 is methylated in vivo. .; Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly (PubMed:20421892, PubMed:26165940). Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble (By similarity). .

Protein cellular localization

Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle pole . Note=Peripheral membrane protein associated with cis-Golgi stacks (By similarity). Associates with the mitotic spindle during mitosis (PubMed:26165940). .

Research area

All research areas>Transport and Trafficking>GM130
(View all antibody categories related to Transport and Trafficking)

Note

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Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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