Anti-NOL3 Antibody

$99.00$319.00

Reactivity: Human
Applications: ELISA,IHC
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-NOL3 Antibody (CSB-PA015921DSR2HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

Nucleolar protein 3Imported Antibody,Apoptosis repressor with CARD Antibody,NOL3 Antibody

More alternative names for the antibody
Apoptosis repressor with CARD antibody|ARC antibody|Muscle enriched cytoplasmic protein antibody|Muscle-enriched cytoplasmic protein antibody|MYC antibody|MYP antibody|Nol3 antibody|NOL3_HUMAN antibody|NOP antibody|Nop30 antibody|Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody|Nucleolar protein 3 antibody|Nucleolar protein of 30 kDa antibody
Anti-Apoptosis repressor with CARD antibody (ab126238)
Close sc-390949|sc-166461|sc-17839|sc-6382|sc-55475|sc-374177|

Recommended applications: ELISA, IHC

Recommended dilution: Recommended dilution:IHC:1:20-1:200

Recommended protocols: check protocols

Name

Anti-NOL3 Antibody

Catalogue No.

CSB-PA015921DSR2HU

Reactivity

Human

Immunogen

Recombinant human Nucleolar protein 3 protein (1-208AA)

Host

Rabbit

Applications

ELISA, IHC

Recommended dilution

Recommended dilution:IHC:1:20-1:200

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

22 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:8996( Human), Entrez Gene:78688( Mouse), Omim:605235( Human), SwissProt:O60936( Human), SwissProt:Q9D1X0( Mouse), Unigene:513667( Human), Unigene:204876( Mouse), Unigene:475715( Mouse)

Protein function

Isoform 1: May be involved in RNA splicing. .; Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors (PubMed:15004034). Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis (PubMed:15509781). Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation (By similarity). Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner (By similarity). Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I (By similarity). Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia (By similarity). Inhibits too myoblast differentiation through caspase inhibition (By similarity). .

Protein tissue specificity

Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

Involvement in disease

Myoclonus, familial cortical (FCM) [MIM:614937]: An autosomal dominant neurologic condition characterized by adult onset of cortical myoclonus manifest as involuntary jerks or movements affecting the face and limbs. Affected individuals can also experience falls without seizure activity or loss of consciousness. . Note=The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

CARD is critical for both extrinsic and intrinsic apoptotic pathways (By similarity). CARD domain mediates a protective effect against myocardial ischemia/reperfusion, oxidative stress and TNF-induced necrosis (PubMed:15004034) (By similarity). The calcium binding domain plays a protective role in calcium-mediated cell death (PubMed:15509781).

Protein post-translational modifications

Phosphorylation at Thr-149 is required for its antiapoptotic effect by blocking death-inducing signaling complex death-inducing signaling complex (DISC) activity through the control of interaction with CASP8. Phosphorylation at Thr-149 results in translocation to mithochondria and this translocation enables the binding to CASP8. Dephosphorylated at Thr-149 by calcineurin; doesn’t inhibit the association between FADD and CASP8 and the consequent apoptosis. .; Polyubiquitinated by MDM2; promoting proteasomal-dependent degradation in response to apoptotic stimuli. .

Protein cellular localization

Isoform 1: Nucleus, nucleolus . Note=The SR-rich C-terminus mediates nuclear localization. .; Isoform 3: Cytoplasm .; Isoform 2: Cytoplasm . Mitochondrion . Sarcoplasmic reticulum . Membrane ; Lipid-anchor . Note=Phosphorylation at Thr-149 results in translocation to mithochondria. Colocalized with mitochondria in response to oxidative stress. .

Research area

All research areas>Tumor Suppressor/Apoptosis>Apoptosis Repressor with CARD
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

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Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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