Anti-PRKAA2 Antibody

$99.00$319.00

Reactivity: Human
Applications: ELISA,WB
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-PRKAA2 Antibody (CSB-PA805325ESR1HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

AMPK subunit alpha-2 Antibody,Acetyl-CoA carboxylase kinase Antibody,ACACA kinase Antibody,Hydroxymethylglutaryl-CoA reductase kinase Antibody,HMGCR kinase Antibody,PRKAA2 Antibody,AMPK Antibody,AMPK2 Antibody

More alternative names for the antibody
5′-AMP-activated protein kinase catalytic subunit alpha-2 antibody|AAPK2_HUMAN antibody|ACACA kinase antibody|Acetyl-CoA carboxylase kinase antibody|AMPK alpha 2 chain antibody|AMPK subunit alpha-2 antibody|AMPK2 antibody|AMPKa2 antibody|AMPKalpha2 antibody|HMGCR kinase antibody|Hydroxymethylglutaryl-CoA reductase kinase antibody|PRKAA antibody|PRKAA2 antibody|Protein kinase AMP activated alpha 2 catalytic subunit antibody|Protein kinase AMP activated catalytic subunit alpha 2 antibody
Anti-AMPK alpha 2 antibody (ab3760)
Close sc-19129|sc-19131|sc-19133|

Recommended applications: ELISA, WB

Recommended dilution: Recommended dilution:WB:1:500-2000

Recommended protocols: check protocols

Name

Anti-PRKAA2 Antibody

Catalogue No.

CSB-PA805325ESR1HU

Reactivity

Human

Immunogen

Recombinant human 5′-AMP-activated protein kinase catalytic subunit alpha-2 protein (343-552AA)

Host

Rabbit

Applications

ELISA, WB

Recommended dilution

Recommended dilution:WB:1:500-2000

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

62 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:5563( Human), Entrez Gene:108079( Mouse), Entrez Gene:397504( Pig), Entrez Gene:78975( Rat), Omim:600497( Human), SwissProt:P54646( Human), SwissProt:Q8BRK8( Mouse), SwissProt:Q28948( Pig), SwissProt:Q09137( Rat), Unigene:437039( Human), Unigene:48638( Mouse), Unigene:64583( Rat)

Protein function

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin receptor/INSR internalization (PubMed:25687571). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates ‘Ser-36’ of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regulation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1 (By similarity). .

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.

Protein post-translational modifications

Ubiquitinated. .; Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1). .

Protein cellular localization

Cytoplasm . Nucleus . Note=In response to stress, recruited by p53/TP53 to specific promoters.

Research area

All research areas>Kinases and Phosphatases>AMPK
(View all antibody categories related to Kinases and Phosphatases)

Note

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Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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