Anti-PRKAG3 Antibody

$99.00$319.00

Reactivity: Human
Applications: ELISA,WB
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-PRKAG3 Antibody (CSB-PA883379ESR1HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

AMPK gamma3 Antibody,AMPK subunit gamma-3 Antibody,PRKAG3 Antibody,AMPKG3 Antibody

More alternative names for the antibody
5 AMP activated protein kinase subunit gamma 3 antibody|5”-AMP-activated protein kinase subunit gamma-3 antibody|AAKG3_HUMAN antibody|AMPK gamma 3 chain antibody|AMPK gamma3 antibody|AMPK subunit gamma-3 antibody|AMPKG3 antibody|PRKAG3 antibody|Protein kinase AMP activated gamma 3 non catalytic subunit antibody
Anti-AMPK gamma 3 antibody (ab203214)
Close sc-19145|sc-19146|sc-130687|sc-20166|

Recommended applications: ELISA, WB

Recommended dilution: Recommended dilution:WB:1:500-2000

Recommended protocols: check protocols

Name

Anti-PRKAG3 Antibody

Catalogue No.

CSB-PA883379ESR1HU

Reactivity

Human

Immunogen

Recombinant human 5′-AMP-activated protein kinase subunit gamma-3 protein (1-210AA)

Host

Rabbit

Applications

ELISA, WB

Recommended dilution

Recommended dilution:WB:1:500-2000

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

54 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:53632( Human), Entrez Gene:241113( Mouse), Entrez Gene:301518( Rat), Omim:604976( Human), SwissProt:Q9UGI9( Human), SwissProt:Q8BGM7( Mouse), Unigene:591634( Human), Unigene:166501( Mouse), Unigene:453463( Mouse), Unigene:149163( Rat)

Protein function

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. .

Protein tissue specificity

Skeletal muscle, with weak expression in heart and pancreas.

Protein sequence and domain

Belongs to the 5′-AMP-activated protein kinase gamma subunit family. The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.; The 4 CBS domains mediate binding to nucleotides. Of the 4 potential nucleotide-binding sites, 3 are occupied, designated as sites 1, 3, and 4 based on the CBS modules that provide the acidic residue for coordination with the 2′- and 3′-hydroxyl groups of the ribose of AMP. Of these, site 4 appears to be a structural site that retains a tightly held AMP molecule (AMP 3). The 2 remaining sites, 1 and 3, can bind either AMP, ADP or ATP. Site 1 (AMP, ADP or ATP 1) is the high-affinity binding site and likely accommodates AMP or ADP. Site 3 (AMP, ADP or ATP 2) is the weakest nucleotide-binding site on the gamma subunit, yet it is exquisitely sensitive to changes in nucleotide levels and this allows AMPK to respond rapidly to changes in cellular energy status. Site 3 is likely to be responsible for protection of a conserved threonine in the activation loop of the alpha catalytic subunit through conformational changes induced by binding of AMP or ADP.

Protein post-translational modifications

Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. .

Research area

All research areas>Kinases and Phosphatases>AMPK
(View all antibody categories related to Kinases and Phosphatases)

Note

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Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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