Anti-PRKG1 Antibody

$99.00$319.00

Reactivity: Human,Mouse
Applications: ELISA,WB
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-PRKG1 Antibody (CSB-PA619797ESR2HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

cGK 1 Antibody,cGK1 Antibody,cGMP-dependent protein kinase I Antibody,cGKI Antibody,PRKG1 Antibody,PRKG1B Antibody, PRKGR1A Antibody, PRKGR1B Antibody

More alternative names for the antibody
cGK 1 antibody|cGK1 antibody|cGKI alpha antibody|cGKI antibody|cGKI beta antibody|cGMP-dependent protein kinase 1 antibody|cGMP-dependent protein kinase I antibody|DKFZp686K042 antibody|FLJ36117 antibody|KGP1_HUMAN antibody|KGPB antibody|MGC71944 antibody|pkg antibody|PRKG1 antibody|PRKG1B antibody|PRKGR1A antibody|PRKGR1B antibody
Anti-cGKI antibody (ab90502)
Close sc-10338|sc-393987|sc-10335|sc-271765|sc-271766|sc-25429|sc-10343|sc-10342|sc-10341|

Recommended applications: ELISA, WB

Recommended dilution: Recommended dilution:WB:1:500-2000

Recommended protocols: check protocols

Name

Anti-PRKG1 Antibody

Catalogue No.

CSB-PA619797ESR2HU

Reactivity

Human, Mouse

Immunogen

Recombinant human cGMP-dependent protein kinase 1 protein (1-300AA)

Host

Rabbit

Applications

ELISA, WB

Recommended dilution

Recommended dilution:WB:1:500-2000

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

76 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:282004( Cow), Entrez Gene:5592( Human), Entrez Gene:19091( Mouse), Entrez Gene:54286( Rat), Omim:176894( Human), SwissProt:P00516( Cow), SwissProt:Q13976( Human), SwissProt:P0C605( Mouse), Unigene:407535( Human), Unigene:381170( Mouse), Unigene:204724( Rat)

Protein function

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle. .

Protein tissue specificity

Primarily expressed in lung and placenta. .

Involvement in disease

Aortic aneurysm, familial thoracic 8 (AAT8) [MIM:615436]: A disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as ‘medial necrosis’ or ‘Erdheim cystic medial necrosis’ in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. . Note=The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Composed of an N-terminal leucine-zipper domain followed by an autoinhibitory domain, which mediate homodimer formation and inhibit kinase activity, respectively. Next, two cGMP-binding domains are followed by the catalytic domain at the C-terminus. Binding of cGMP to cGMP-binding domains results in a conformational change that activates kinase activity by removing the autoinhibitory domain from the catalytic cleft leaving the catalytic domain free to phosphorylate downstream substrates. Isoforms alpha and beta have identical cGMP-binding and catalytic domains but differ in their leucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.; Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

Protein post-translational modifications

Autophosphorylation increases kinase activity.; 65 kDa monomer is produced by proteolytic cleavage. .

Protein cellular localization

Cytoplasm . Note=Colocalized with TRPC7 in the plasma membrane. .

Research area

All research areas>Kinases and Phosphatases>cGKI
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus provides antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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