Anti-SETDB1 Antibody

$99.00$319.00

Reactivity: Human,Rat
Applications: ELISA,WB,IHC
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-SETDB1 Antibody (CSB-PA619960ESR1HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

ERG-associated protein with SET domain Antibody,ESET Antibody,Histone H3-K9 methyltransferase 4 Antibody,H3-K9-HMTase 4 Antibody,Lysine N-methyltransferase 1E Antibody,SET domain bifurcated 1 Antibody,SETDB1 Antibody

More alternative names for the antibody
AU022152 antibody|EC 2.1.1.43 antibody|ERG-associated protein with SET domain antibody|ESET antibody|H3 K9 HMTase 4 antibody|H3-K9-HMTase 4 antibody|H3-K9-HMTase4 antibody|Histone H3 K9 methyltransferase 4 antibody|Histone H3-K9 methyltransferase 4 antibody|Histone-lysine N-methyltransferase SETDB1 antibody|KG1T antibody|KIAA0067 antibody|KMT1E antibody|Lysine N-methyltransferase 1E antibody|MGC90670 antibody|mKIAA0067 antibody|SET domain bifurcated 1 antibody|Set domain protein, bifurcated, 1 antibody|SETB1_HUMAN antibody|Setdb1 antibody
Anti-KMT1E / SETDB1 antibody – ChIP Grade (ab12317)
Close sc-166621|sc-271553|sc-130437|sc-271488|sc-66884|

Recommended applications: ELISA, WB, IHC

Recommended dilution: Recommended dilution:WB:1:200-1000,IHC:1:20-1:200

Recommended protocols: check protocols

Name

Anti-SETDB1 Antibody

Catalogue No.

CSB-PA619960ESR1HU

Reactivity

Human, Rat

Immunogen

Recombinant human Histone-lysine N-methyltransferase SETDB1 protein (1-397AA)

Host

Rabbit

Applications

ELISA, WB, IHC

Recommended dilution

Recommended dilution:WB:1:200-1000,IHC:1:20-1:200

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

143 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:9869( Human), Entrez Gene:84505( Mouse), Omim:604396( Human), SwissProt:Q15047( Human), SwissProt:O88974( Mouse), Unigene:643565( Human)

Protein function

Histone methyltransferase that specifically trimethylates ‘Lys-9’ of histone H3. H3 ‘Lys-9’ trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 ‘Lys-9’ trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone ‘Lys-9′ trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3’-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). .

Protein tissue specificity

Widely expressed. High expression in testis.

Protein sequence and domain

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.; Isoform 2 lacks all domains required for histone methyltransferase activity.; In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.

Protein cellular localization

Nucleus. Chromosome. Note=Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.

Research area

All research areas>Transcription Regulators>ESET
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus provides antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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