Anti-SIAH1 Antibody

$99.00$319.00

Reactivity: Human
Applications: ELISA,IHC
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-SIAH1 Antibody (CSB-PA818230ESR1HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

Seven in absentia homolog 1 Antibody,Siah-1 Antibody,Siah-1a Antibody,SIAH1 Antibody,HUMSIAH Antibody

More alternative names for the antibody
E3 ubiquitin protein ligase SIAH2 antibody|E3 ubiquitin-protein ligase SIAH1 antibody|hSIAH1 antibody|hSiah2 antibody|HUMSIAH antibody|Seven in absentia homolog 1 (Drosophila) antibody|Seven in absentia homolog 1 antibody|Seven in Absentia Homolog 2 antibody|SIAH 1 antibody|Siah 1a antibody|Siah 2 antibody|Siah-1 antibody|Siah-1a antibody|Siah1 antibody|SIAH1_HUMAN antibody|Ubiquitin ligase SIAH1 antibody|Ubiquitin Ligase Siah2 antibody
Anti-SIAH1/2 antibody (ab209551)
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Recommended applications: ELISA, IHC

Recommended dilution: Recommended dilution:IHC:1:20-1:200

Recommended protocols: check protocols

Name

Anti-SIAH1 Antibody

Catalogue No.

CSB-PA818230ESR1HU

Reactivity

Human

Immunogen

Recombinant human E3 ubiquitin-protein ligase SIAH1 protein (1-313AA)

Host

Rabbit

Applications

ELISA, IHC

Recommended dilution

Recommended dilution:IHC:1:20-1:200

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

31 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:374076( Chicken), Entrez Gene:429164( Chicken), Entrez Gene:6477( Human), Entrez Gene:6478( Human), Entrez Gene:20437( Mouse), Entrez Gene:20439( Mouse), Entrez Gene:140593( Rat), Entrez Gene:140941( Rat), Entrez Gene:378728( Zebrafish), Entrez Gene:80955( Zebrafish), Omim:602212( Human), Omim:602213( Human), SwissProt:O43255( Human), SwissProt:Q8IUQ4( Human), SwissProt:P61092( Mouse), SwissProt:Q06986( Mouse), SwissProt:Q8R4T2( Rat), SwissProt:Q920M9( Rat), SwissProt:378728( Zebrafish), SwissProt:Q7SYL3( Zebrafish), SwissProt:Q7ZVG6( Zebrafish), Unigene:706828( Human), Unigene:73937( Rat), Unigene:5064( Zebrafish)

Protein function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. .

Protein tissue specificity

Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas. .

Protein sequence and domain

Belongs to the SINA (Seven in absentia) family. The RING-type zinc finger domain is essential for ubiquitin ligase activity.; The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.

Protein post-translational modifications

Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2. .

Protein cellular localization

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear.

Research area

All research areas>Tumor Suppressor/Apoptosis>SIAH
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus provides antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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