Anti-SPAG5 Antibody

$99.00$319.00

Reactivity: Human
Applications: ELISA,IHC
Conjugation: Various
Supplier: CUSABIO BIOTECH CO.

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Rabbit Anti-SPAG5 Antibody (CSB-PA853491ESR2HU)

Supplier: CUSABIO BIOTECH CO.

Alternative names:

Astrin Antibody,Deepest Antibody,Mitotic spindle-associated protein p126 Antibody,MAP126 Antibody,SPAG5 Antibody

More alternative names for the antibody
Astrin antibody|Mitotic spindle-associated protein p126 antibody|SPAG 5 antibody|SPAG5 antibody|SPAG5_HUMAN antibody|Sperm associated antigen 5 antibody|Sperm tail protein Spag 5 antibody|Sperm tail protein Spag5 antibody|Sperm-associated antigen 5 antibody
Anti-MAP126 antibody (ab214182)
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Recommended applications: ELISA, IHC

Recommended dilution: Recommended dilution:IHC:1:20-1:200

Recommended protocols: check protocols

Name

Anti-SPAG5 Antibody

Catalogue No.

CSB-PA853491ESR2HU

Reactivity

Human

Immunogen

Recombinant human Sperm-associated antigen 5 protein (1-300AA)

Host

Rabbit

Applications

ELISA, IHC

Recommended dilution

Recommended dilution:IHC:1:20-1:200

Clonality

Polyclonal

Conjugation

unconjugated

Isotype

IgG

Molecular weight

134 kDa

Purification

Antigen Affinity Purified

Storage

Shipped at 4 Celcius Degree. Upon delivery aliquot and store at -20 Celcius Degree or -80 Celcius Degree. Avoid repeated freeze.

Database links

Entrez Gene:10615( Human), Entrez Gene:54141( Mouse), Entrez Gene:252918( Rat), Omim:615562( Human), SwissProt:Q96R06( Human), SwissProt:Q7TME2( Mouse), Unigene:514033( Human), Unigene:24250( Mouse), Unigene:154430( Rat)

Protein function

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457). .

Protein tissue specificity

Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon. .

Protein post-translational modifications

Phosphorylated by AURKA. .

Protein cellular localization

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite . Note=Colocalizes with PCM1 at centriolar satellites throughout the cell cycle (PubMed:26297806). In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (By similarity). In non-mitotic non-stressed cells, shows a microtubuli pattern. In arsenite-stressed cells, accumulates in stress granules. .

Research area

All research areas>Signaling Intermediates>SPAG
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus provides antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

CUSABIO BIOTECH CO.

Product type

Primary antibody

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