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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sensitivity||< 0.6 ng/ml|
|Sample Type||Serum, heparin Plasma, urine, breast milk, cell culture media|
|Sample Volume||100 ul|
|Full Name||adiponectin, C1Q and collagen domain containing|
|Alternate Names||Check alternative names for the Human Adiponectin ELISA Kit Expand
adipocyte-specific secretory protein Elisa kits|Adiponectin Elisa kits|Adiponectin precursor Elisa kits|adiponectin, C1Q and collagen domain containing Elisa kits|Adipoq Elisa kits|Adipose most abundant gene transcript 1 Elisa kits|Adipose most abundant gene transcript 1 protein Elisa kits|Adipose specific collagen like factor Elisa kits|ADIPQTL1 Elisa kits|ADPN Elisa kits|APM 1 Elisa kits|apM-1 Elisa kits|APM1 Elisa kits|C1q and collagen domain-containing protein Elisa kits|GBP28 Elisa kits|Gelatin binding protein Elisa kits|Gelatin binding protein 28 Elisa kits|Gelatin-binding protein Elisa kits
|Assay Time||1 h, 30 min (RT/shaking), 15 min (RT)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Human Adiponectin ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
< 0.6 ng/ml
|Sample type|| |
Serum, heparin Plasma, urine, breast milk, cell culture media
|Sample Volume|| |
|Target name|| |
|Assay time|| |
1 h, 30 min (RT/shaking), 15 min (RT)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Adiponectin is a 30kDa protein which percentage in serum proteins is 0.01%. It is mainly synthesized by Adipocytes, but also muscle cells and hepatocytes have the ability to synthesize Adiponectin. Until now, IGF-I is the only known natural inductor of the synthesis. It consists of a Collagen-like N-terminal and a globular C-terminal domain. In vivo Adiponectin appears with different oligomers. Beside the trimer and ditrimer also high molecular multimers exist. Up to now two different receptors are known, both receptors are ubiquitary expressed, though the distribution in the tissues varies. The Adiponectin Receptor 1 (AdipoR1) is especially in muscle- and AdipoR2 in liver tissue synthesized. The significance for the human organism is not clear until now. First studies show, that adiponectin correlates negatively with BMI and thus it could have relevance for the energy metabolism for example through the regulation of fatty acid oxidation. Beside the correlation with BMI, Adiponectin level is associated with the Insulin-Resistance and so also linked with Type II Diabetes. Adiponectin is associated also with glucose- und lipometabolism. The formerly proposed diagnostic value of the high molecular weight form of adiponectin was not verified using a commercially available testsystem for the determination of HMW adiponectin. Blueher et al. clearly demonstrate that regarding the diagnosis of insulin resistance, measured by whole body glucose uptake below 40 ?mol/kg*min, total adiponectin as determined with this testkit, is with an area of 0.92 under the receiver-operating curve, of greater diagnostic value. Furthermore it is involved in inflammatory processes and therewith it is of importance for appearance of arteriosclerosis and coronaritis, thus the determination of Adiponectin level in plasma could serve to estimate the risk of coronary disease. Beside this Adiponectin influences further physiological processes as for example the angiogenesis.
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