Human Androstenedione ELISA Kit (ARG80858)

$599.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Androstenedione
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 5 pg/ml
Detection Range
Sample Type saliva
Sample Volume 50 ul
Precision

CV: <10%

Full Name Androstenedione
Alternate Names Check alternative names for the Human Androstenedione ELISA Kit

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Application Instructions

 

Assay Time 1 h (37 Celcius degree), 15 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Androstenedione ELISA Kit  Download
size

96 wells

Cat No.

ARG80858

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

5 pg/ml

Sample type

saliva

Sample Volume

50 ul

Precision

CV: <10%

Target name

Androstenedione

Assay time

1 h (37 Celcius degree), 15 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Androstenedione (also known as 4-androstenedione) is a 19-carbon steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol. It is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens. Androstenedione originates either from the conversion of dehydroepiandrosterone or from 17-hydroxyprogesterone. It is further converted to either testosterone or estrone. The production of adrenal androstenedione is governed by ACTH, while production of gonadal androstenedione is under control by gonadotropins. In premenopausal women the adrenal glands and ovaries each produce about half of the total androstendione (about 3 mg/day). After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary. Increased Androstenedione levels often are seen in PCOS.

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