Human C Peptide ELISA Kit (ARG80782)

$599.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name C Peptide
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.064 ng/ml
Detection Range
Sample Type serum, plasma, urine
Sample Volume 100 ul
Precision

CV: <10%

Full Name insulin
Alternate Names Check alternative names for the Human C Peptide ELISA Kit

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Application Instructions

 

Assay Time 5 min, 3 h, 1 h, 30 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human C Peptide ELISA Kit  Download
size

96 wells

Cat No.

ARG80782

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.064 ng/ml

Sample type

serum, plasma, urine

Sample Volume

100 ul

Precision

CV: <10%

Target name

C Peptide

Assay time

5 min, 3 h, 1 h, 30 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Insulin is synthesized in the pancreatic beta cells as a 6000 MW component of an 86 amino acid polypeptide called proinsulin. Proinsulin is subsequently cleaved enzymatically, releasing insulin into the circulation along with a residual 3000 MW fragment called connection (“C”) peptide, so-named because it connects A and B chains of insulin within the proinsulin molecule. Human C-Peptide, a 31 amino acid residue peptide, has a molecular mass of approximately 3000 daltons. C-Peptide has no metabolic function. However, since C-Peptide and insulin are secreted in equimolar amounts, the immunoassay of C?Peptide permits the quantitation of insulin secretion. This is the reason for the clinical interest of serum and urinary determinations of C-Peptide. Moreover, C-Peptide measurement has several advantages over immunoassays of insulin. The half-life of C-Peptide in the circulation is between two and five times longer than that of insulin. Therefore, C-Peptide levels are a more stable indicator of insulin secretion than the more rapidly changing levels of insulin. A very clear practical advantage of C-Peptide measurement arising from its relative metabolic inertness as compared to insulin is that C-Peptide levels in peripheral venous blood are about 5-6 times greater than insulin levels. Also, relative to an insulin assay, the C-Peptide assay’s advantage is its ability to distinguish endogenous from injected insulin. Thus, low C-Peptide levels are to be expected when insulin is diminished (as in insulin-dependent diabetes) or suppressed (as a normal response to exogenous insulin), whereas elevated C-Peptide levels may result from the increased beta-cell activity observed in insulinomas. C-Peptide has also been measured as an additional means for evaluating glucose tolerance and glibenclamide glucose tests. C-Peptide levels are in many ways a better measurement of endogenous insulin secretion than peripheral insulin levels. C-Peptide may be measured in either blood or urine. With improved sensitive C-Peptide immunoassays, it is now possible to measure C-Peptide values at extremely low levels. The clinical indications for C-Peptide measurement include diagnosis of insulinoma and differentation from factitious hypoglycemia, follow-up of pancreatectomy, and evaluation of viability of islet cell transplants. Recently, these indications have been dramatically expanded to permit evaluation of insulin dependence in maturity onset diabetes mellitus. Clinical Indications for the C-Peptide ELISA – Assessment of residual ?-cell function in diabetics under insulin therapy – Detection and monitoring of the remission phase of type I diabetes – Adjunct in the differential diagnosis between type I (insulin dependent) and type II (non-insulin-dependent) diabetes – Diagnosis of insulin-induced factitious hypoglycemia. – Contribution to the diagnosis of insulinoma (insulin suppression test) – Prognostic index of fetal outcome in pregnant diabetic women – Evaluation of insulin secretion in liver disease – Monitoring of pancreasectomy

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