Human CXCL12 / SDF-1 ELISA Kit (ARG80121)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human beta-ECGF, GRO alpha, GRO beta, GRO gamma, MCP-1, MIP-1 alpha, MIP-1 beta, PTN, RANTES;mouse MIP-1 alpha, MIP-1 beta
Target Name CXCL12 / SDF-1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 60 pg/ml
Detection Range 125-8000 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name chemokine (C-X-C motif) ligand 12
Alternate Names Check alternative names for the Human CXCL12 / SDF-1 ELISA Kit

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human CXCL12 / SDF-1 ELISA Kit  Download
size

96 wells

Cat No.

ARG80121

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

60 pg/ml

Detection range

125-8000 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

CXCL12 / SDF-1

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [provide by uniprot]

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