Human Dihydrotestosterone / DHT ELISA Kit (ARG80838)

$599.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Dihydrotestosterone / DHT
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 7.23 pg/ml
Detection Range
Sample Type serum
Sample Volume 50 ul
Precision

CV: <10%

Full Name Dihydrotestosterone (DHT)
Alternate Names Check alternative names for the Human Dihydrotestosterone / DHT ELISA Kit

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Application Instructions

 

Assay Time 60, 15 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Dihydrotestosterone / DHT ELISA Kit  Download
size

96 wells

Cat No.

ARG80838

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

7.23 pg/ml

Sample type

serum

Sample Volume

50 ul

Precision

CV: <10%

Target name

Dihydrotestosterone / DHT

Assay time

60, 15 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

5 alpha-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Some metabolites are formed and some are returned to the circulation before renal excretion. Therefore, elimination of steroids from the body is done through the urine. Clinical Trends: In Klinefelter’s syndrome the DHT level is much lower than that found in normal men. In idiopathic hirsutism about 40% of the patients have an increased level of DHT. In polycystic ovaries (PCO) about 35% of the patients have an increased DHT level. The DHT level in young people is much higher than those found in normal older people, hence androgen production increases at puberty which gives rise to masculinizing characteristics. It has been demonstrated that the human testes produce DHT, which appears to originate in the seminiferous tubules. Therefore in tubular damage the production of DHT is impaired, which causes a decrease in the levels of plasma DHT (patients with germinal cell aplasia and azoospermia). There is a very low level of plasma DHT in patients with anorchia. It has been reported that in some prostate cancer (especially in stage D) the determination of DHT could be useful in predicting the response to anti-androgen therapy.

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