Human Estriol (unconjugated) ELISA Kit (ARG80842)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Estriol
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.02 ng/ml
Detection Range
Sample Type serum
Sample Volume 10 ul
Precision

CV: <10%

Full Name Estriol, unconjugated
Alternate Names Check alternative names for the Human Estriol (unconjugated) ELISA Kit

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Application Instructions

 

Assay Time 1h, 30 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Estriol (unconjugated) ELISA Kit  Download
size

96 wells

Cat No.

ARG80842

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.02 ng/ml

Sample type

serum

Sample Volume

10 ul

Precision

CV: <10%

Target name

Estriol

Assay time

1h, 30 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Estriol (E3) is the major estrogen formed by the fetoplacental unit during pregnancy. Unconjugated E3 passes through the placenta into the maternal circulation, where it is rapidly converted into glucuronide and sulfate derivatives to facilitate its excretion. The half-life of estriol in the maternal bloodstream is only 20-30 minutes. Its measurement, therefore offers a convenient and quick evaluation of current fetal status. Plasma estriol levels increase steadily throughout pregnancy and most rapidly during the third trimester (28-40 weeks). A sudden decrease in fetoplacental E3 production will result in a rapid fall in unconjugated E3 in the maternal serum. There are several potential advantages to measuring unconjugated E3 rather than total serum or urinary E3. Unconjugated estriol levels are free from effects related to maternal renal or hepatic disease, and are not altered by the administration of certain antibiotics. Unconjugated E3 more accurately reflects fetal outcome in diabetic pregnancies – and since no hydrolysis of unconjugated E3 is required, a more rapid tumaround for the test result is possible.

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