Human Free Triiodothyronine (fT3) ELISA Kit (ARG80888)

$599.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Triiodothyronine
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.05 pg/ml
Detection Range
Sample Type serum
Sample Volume 50 ul
Precision

CV: <10%

Full Name Free Triiodothyronine (fT3)
Alternate Names Check alternative names for the Human Free Triiodothyronine (fT3) ELISA Kit

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Application Instructions

 

Assay Time 60, 20 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Free Triiodothyronine (fT3) ELISA Kit  Download
size

96 wells

Cat No.

ARG80888

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.05 pg/ml

Sample type

serum

Sample Volume

50 ul

Precision

CV: <10%

Target name

Triiodothyronine

Assay time

60, 20 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

L-Triiodothyronine, a thyroid hormone, circulates in blood almost completely bound (>99.5%) to carrier proteins. The main transport protein is thyroxine-binding globulin (TBG). However, only the free (unbound) portion of triiodothyronine is believed to be responsible for the biological action. Furthermore, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In individuals with normal thyroid function, as the concentrations of the carrier proteins change, the total T3 levels change in consert so that the free thriiodothyronine (fT3) concentration remains constant. Thus, measurements of fT3 concentrations correlate more reliably with clinical status than total triiodothyronine levels. For example, the increase in total triiodothyronine levels associated with pregnancy, oral contraceptives, and estrogen therapy result in higher total T3 levels while the fT3 concentration remains basically unchanged. This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations in a direct determination of fT3

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