Human Ghrelin ELISA Kit (ARG80942)

$539.00

Size: 96 wells
Conjugation:
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Human Ghrelin
Conjugation
Conjugation Note
Sensitivity 4 ng/ml
Detection Range 7.8 – 500 ng/ml
Sample Type Serum, Plasma and Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name ghrelin/obestatin prepropeptide
Alternate Names Check alternative names for the Human Ghrelin ELISA Kit

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Application Instructions

 

Assay Time ~200 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Ghrelin ELISA Kit  Download
size

96 wells

Cat No.

ARG80942

Sensitivity

4 ng/ml

Detection range

7.8 – 500 ng/ml

Sample type

Serum, Plasma and Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

Human Ghrelin

Assay time

~200 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

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