Human hGH ELISA Kit (ARG80824)

$599.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name hGH
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.2 ?IU/ml
Detection Range
Sample Type serum, plasma
Sample Volume 50 ul
Precision

CV: <10%

Full Name growth hormone 1
Alternate Names Check alternative names for the Human hGH ELISA Kit

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Application Instructions

 

Assay Time 1 h, 15 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human hGH ELISA Kit  Download
size

96 wells

Cat No.

ARG80824

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.2 ?IU/ml

Sample type

serum, plasma

Sample Volume

50 ul

Precision

CV: <10%

Target name

hGH

Assay time

1 h, 15 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

hGH is a polypeptide hormone (molecular weight 21,500 Da) produced by the acidophil cells of the anterior pituitary under the control of two main substances from the median eminence : Growth-hormone Releasing Factor (GRF) and an inhibitory agent, somatostatin. Dopaminergic, adrenergic and serotoninergic neuroendocrine pathways also play an important role in the control of hGH secretion. Excitatory stimuli of hGH secretion include hypoglycemia, exercise, fasting, meals with a high protein content, deep sleep, stress, glucagon, L Dopa, amino acids, etc. Inhibitory stimuli include glucose, cortisol, hGH and free fatty acids. Because of its short plasma half life (¡À25 minutes) and of the frequent excitatory or inhibitory stimuli, hGH displays frequent and large variations of concentration in serum. One of the main physiological functions of hGH is to act on the liver and other tissues to produce Somatomedins, which in turn induce growth by direct action on target tissues. In contrast to hGH, the concentration of somatomedin in serum is kept stable by virtue of being largely bound to circulating plasma proteins. Growth retardation hGH hyposecretion is one of the various causes of small stature in children. Serum hGH measurement with a highly sensitive assay, especially following a provocative stimulus (absence of response), is an important way to establish this diagnosis because this group of patients can be treated by administration of hGH. Hypopituitarism Serum hGH measurement is also an index of pituitary function when hypopituitarism (either idiopathic or due to tumour and surgery) is suspected. Gigantism and acromegaly Serum hGH measurement, especially following a provocative inhibitory test (absence of response), is an important way to establish the diagnosis of hGH hypersecretion due to acidophilic pituitary tumour. This results in gigantism in children and acromegaly in adults. Both of these disorders may be treated by surgery or radiation.

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