Human IgG4 ELISA Kit (ARG80175)

$539.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IgG1, IgG2, IgG3
Target Name IgG4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 8 ng/ml
Detection Range 15.6-1000 ng/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name
Alternate Names Check alternative names for the Human IgG4 ELISA Kit

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human IgG4 ELISA Kit  Download
size

96 wells

Cat No.

ARG80175

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

8 ng/ml

Detection range

15.6-1000 ng/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

IgG4

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

IgG4 is present in very low levels in children younger than 10 years of age, so IgG4 deficiencies are not usually diagnosed before age 10. IgG4 may be undetectable in the serum of many ¡°normal¡± adult individuals, and therefore low IgG4 alone is insufficient evidence of an antibody deficiency disorder requiring Ig replacement. IgG4¨Crelated systemic disease?(IgG4-RSD), also known as?hyper-IgG4 disease?and?IgG4-related disease?is a disease in which inflammatory cells cause?fibrosis, the deposition of?connective tissue, in one or more organs. The disease is so named because the antibody subtype?IgG4?can be detected on tissue samples and often at elevated levels in the bloodstream. The association with IgG4 is a relatively recent finding, and the condition has been described under numerous other names in the past. Diseases such asautoimmune pancreatitis,?retroperitoneal fibrosis,?mediastinal fibrosis,?Riedel’s thyroiditis,?Mikulicz’s syndrome,?Kuttner’s tumor?and?inflammatory pseudotumor?are now regarded as forms of IgG4-RSD.

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