Human IL10 ELISA Kit (ARG80828)

$899.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name IL10
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 1.6 pg/ml
Detection Range
Sample Type serum
Sample Volume 100 ul
Precision

CV: <10%

Full Name interleukin 10
Alternate Names Check alternative names for the Human IL10 ELISA Kit

Expand

CSIF Elisa kits|Cytokine synthesis inhibitory factor Elisa kits|GVHDS Elisa kits|IL 10 Elisa kits|IL-10 Elisa kits|IL10 Elisa kits|IL10_HUMAN Elisa kits|IL10A Elisa kits|Interleukin 10 Elisa kits|Interleukin-10 Elisa kits|MGC126450 Elisa kits|MGC126451 Elisa kits|T-cell growth inhibitory factor Elisa kits|TGIF Elisa kits


Application Instructions

 

Assay Time 2 h, 2 h, 30 min (RT/shaking)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human IL10 ELISA Kit  Download
size

96 wells

Cat No.

ARG80828

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

1.6 pg/ml

Sample type

serum

Sample Volume

100 ul

Precision

CV: <10%

Target name

IL10

Assay time

2 h, 2 h, 30 min (RT/shaking)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Biological activities Human interleukin-10 (IL-10) is a 19 kDA lymphokine produced by T helper lymphocytes,by monocytes, macrophages and B-lymphocytes. IL-10 was first characterized as a cytokine synthesis inhibitory factor (CSIF) able to inhibit cytokine synthesis by TH1 clones activated in the presence of antigen presenting cells. However, in the absence of monocytes, IL-10 directly inhibits the growth of T-cells triggered by immobilized anti-CD3 MoAb. This proliferation inhibition was found to be a result of specific inhibition of IL-2 production by the responding T-cells. In vitro, Il-10 is a very powerful inhibitor of monokines (including TNF-alpha, IL-1, IL-6 and IL-8) produced by LPS-activated monocytes and macrophages. The addition of IL-10 to B lymphocytes results in limited cell proliferation but most importantly in very high immunoglobulin production, a result of the transformation of B-cells into plasma cells. Finally, natural killer (NK) cells appear to be another target for the anti-inflammatory properties of IL-10. Indeed, recent data have shown that IL-10 can inhibit antigen induced IFN-gamma production by NK-cells by inhibiting not only production but also the stimulatory effects of IL-12 and TNF on IFN-gamma production. Clinical application So far, circulating levels of IL-10 have been found in serum of patients suffering of Non-Hodgkin’s lymphoma, multiple myeloma, cerebral malaria or septic shock.

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